Sternvolmer quenching constant value for zniicpc is determined. The highest specific growth rate, cell concentration and phycocyanin production were respectively 0. Spirulina reduces oxidative stress, boosts your immune system, and improves circulation. Have you ever seen phycocyanin content higher than 20% ww and what about the cn ratio to improve its synthesis. Phycocyanin is an important commercially available blue food colorant. In the absence of culture aeration in the conical flasks, a maximum biomass concentration of 3. Therefore, ilsbased atps was an effective method for partitioning and recovery of. Phycocyanin in bac enhances the immune system the magic.
Phycocyanin significantly inhibited the growth of k562cells in a dosedependent manner. Keywords phycocyanin extraction, spirulina platensis, bluegreen algae a wide range of phycocyanin extraction methods were studied for the processing of spirulina biomass. Proteins isolated from spirulina have been reported to be quite complex biomacromolecules, likely to be protein andor proteinpigment phycocyanin complexes rather than individual protein molecules. The price of phycocyanin is a vital factor that dictates its marketability. Phycocyanobilin from spirulina the master antioxidant. Supplementary effect of spirulina on hematological status of rats during pregnancy and. Quantitative analysis of cphycocyanin from spirulina.
Spirulina platensis is a blue green algae, which holds marvelous nutritional value and commercial importance. Purified cphycocyanin from spirulina platensis nordstedt. Under favourable conditions for growth, cphycocyanin existed in large excess in the algal cells. Phycocyanobilin from spirulina the master antioxidant mark f. Supplementary effect of spirulina on hematological status of rats during pregnancy and lactation. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of c phycocyanin cpc from spirulina platensis, have been reported, and cpc has important value of development and utilization either as drug or as functional food.
Original article phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. Phycobilisomes of the cyanobacterium spirulina platensis consist of allophycocyanin apc cores surrounded by cphycocyanin cpc on the periphery. Review article medical application of spirulina platensis. The pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of cphycocyanin cpc from spirulina platensis, have. Phycocyanin and phycocyanobilin from spirulina platensis. Cphycocyanin is a protein pigment, that is soluble in water. Drying methods were resulted in approximately 50% loss of phycocyanin. Phycocyanin pc is a major light harvesting pigment of cyanobacteria. This work parametrically investigated the autotrophic cultivation of spirulina platensis for enhanced simultaneous co 2 fixation and phycocyanin production using conical flasks and newly designed photobioreactors. Extraction and characterization of phycocyanin from. Apr 02, 2016 phycocyanin is a pigment protein complex that is heavily sought after for its many health benefits.
It is an important constituent of spirulina platensis. Crystal structure of a lightharvesting protein cphycocyanin from. First phase was conducted by adding 150 mgkg, 250 mgkg, 350 mgkg feed phycocyanin. Phycocyanin valuable component of spirulina fresh spirulina. A wide variety of spirulina phycocyanin options are available to you, such as part, packaging, and form. The following evidence was obtained in support of this idea. Cphycocyanin cpc, a phycobiliprotein, is one of the key pigments of spirulina, a microalgae used in many countries as a. The product phycocyanin, produced by aphanizomenon flosaquae and spirulina, is for example used in the food and beverage industry as the natural coloring agent lina blue or exberry shade blue and is found in sweets and ice cream. Spirulina health benefits theres many health benefits associated with spirulina because of the nutrients found in spirulina such as protein, betacarotene, antioxidants, phycocyanin, chlorophyll, and other. Glucose and acetate enhanced cell growth and phycocyanin production of s. Also known as blue spirulina, phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, found abundantly in spirulina and other cyanobacteria, or bluegreen algae as they are often called. Thermal stability improvement of blue colorant cphycocyanin from. Cphycocyanin, a very potent and novel platelet aggregation inhibitor from spirulina platensis. This study aimed to evaluate the performance of phycocyanin.
Phycocyanin spirulina organic phycocyanin spirulina. Sternvolmer quenching constant value for zniicpc is. Cyanobacteria spirulina platensis basic protein cphycocyanin. Cyanobacteria the class of spirulina are commonly thought to be the precursors of all the living, and had risen about 3 billions of years ago. One of the most important requirements to obtain phycobiliproteins from cyanobacterium is optimizing the extraction and purification steps. Cphycocyanin extraction from spirulina platensis wet. Extraction and separation of phycocyanin from spirulina. Effects of spirulina platensis extract on syrian hamster cheek pouch mucosa painted with 7,12dimethylbenzaanthracene.
Cphycocyanin extraction from spirulina platensis wet biomass. One unusual phytonutrient in bluegreen algae such as spirulina is the natural blue protein pigment called phycocyanin. Antiinflammatory and antihyperalgesic activity of c. C phycocyanin from spirulina platensis effectively inhibited ccl4induced lipid peroxidation in rat liver in vivo. When the supply of nitrogen was low, about 3050% of the cphycocyanin disappeared without any effect. Optimization of phycocyanin extraction from spirulina. The use of immunostimulant from phycocyanin of spirulina. Analysis of the antifatigue activity of polysaccharides. Purification and immunomodulating activity of cphycocyanin from. Arthrospira platensis spirulina, a filamentous freshwater planktonic. Cphycocyanin as a storage protein in the bluegreen alga. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when. Cyanobacteria the class of spirulina are commonly thought to be the precursors.
Inhibitory effect of phycocyanin from spirulina platensis. Wt40 phycocyanin extraction in spirulina produced using. Extraction and purification of cphycocyanin from spirulina. A parametric study of key process factors essential for the optimization of spirulina platensis cultivation system to simultaneously capture high levels of co 2 and produce high density biomass and. Also known as blue spirulina, phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, found abundantly in spirulina and other cyanobacteria, or bluegreen algae as. Extraction and separation of phycocyanin from spirulina using. It may be found in concentrations as high as 7 percent in certain bluegreen algae, as compared to 1 percent chlorophyll content most commonly found. Phycocyanin is a pigmentprotein complex synthesized by bluegreen microalgae such as arthrospira spirulina platensis.
Spirulina is a great source of phycocyanin, and its all natural. Phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family. The possibility that cphycocyanin serves as a nitrogen source in spirulina platensis during nitrogen starvation was studied. Spirulina phycobiliproteins as food components and complements. The dark green colour of spirulina comes from the high amount of chlorophyll, plant blood, which is only one molecule different to haemoglobin in human blood. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a.
This pigment is used mainly as natural colouring in food industry. Herein we report an optimization study of various phycocyanin extraction methods from spirulina platensis cyanobacterium biomass dry, frozen and wet. Analysis of the antifatigue activity of polysaccharides from spirulina platensis. Spirulina platensis, a blue green algae, is the richest wholefood source available in nature and it gets its name from the shape of the plant which looks like little spirals. Improving the biomass productivity and phycocyanin. Another plant promoter used for constitutive expression in cyanobacteria is the phycocyanin promoter pc promoter from spirulina platensis strain c1 that is a close to consensus. Medical application of spirulina platensis derived cphycocyanin. This is the pigment that gives bluegreen algae its blue color. Supplementing spirulina will provide you with a good amount of phycocyanin, and ill go over exactly how much phycocyanin you can expect to get. The nutritional aspects of spirulina jacques falquet let your food be your medicine.
Cold water extracts from spirulina platensis were prepared as follows. The best phycocyanin supplement, i present to you spirulina. Wound healing effects of cphycocyanin isolated from spirulina. Spirulina contains unusually high amounts of protein, between 55 and 70 percent by dry weight, depending upon the source phang et al.
Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s. Review article medical application of spirulina platensis derived cphycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3 college of pharmacy, jinan university. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural. Enhancement of biomass and phycocyanin content of spirulina platensis. Processingindependent extracellular production of high purity c.
Therefore, ilsbased atps was an effective method for partitioning and recovery of phycocyanin from spirulina extracts. Jul 16, 2014 spirulina is used as a high quality protein mainly for phycocyanin eriksen 2008, which is a important cyanobacterial accessory pigment having a number of industrial applications. Mahammed ilyas khazi 1, zeliha demirel 2, meltem conk dalay 2. Dec 03, 2012 c phycocyanin, a very potent and novel platelet aggregation inhibitor from spirulina platensis.
Effect of fermentation on enhancing the nutraceutical properties of. Dec 05, 2016 phycocyanin an anticholesterol, antioxidant, an antideposits, regeneration agent and detoxifier. Optimization,purification and characterization of p hycocyanin from spirulina platensis suman saran 1, neha puri 2, nakuleshwar dut jasuja 3, manoj kumar 4 and gaurav sharma 5 1,3,4,5 school of sciences, suresh gyan vihar university, mahal jagatpura, jaipur302017,india. A high phycocyanin spirulina a product of hash biotech labs private limited new age super food nutritional comparision spirulina, a blue green algae, is the richest wholefood. Antioxidant and anticancer activity of spirulina platensis. The interaction of znii ions with cyanobacteria spirulina platensis basic protein cphycocyanin cpc is studied by fluorescence spectroscopy. The dependent variables evaluated were the amount of phycocyanin. May 11, 2016 the pharmacological actions, such as antiinflammation, antioxidation, antitumor, immunological enhancement, and hepatorenal protection of c phycocyanin cpc from spirulina platensis, have been reported, and cpc has important value of development and utilization either as drug or as functional food. Medical application of spirulina platensis derived c. Under the optimum conditions, phycocyanin with a purity of 3. The effect of phycocyanin from spirulinaplatensis on the growth of human chronic myelogenousleukemiablast crisis k562 cells was studied bysemisolid agar assay and cell viability measurement. About 46% of these are herbal extract, 0% are tea extract, and 0% are fruit extract. Phycocyanin content in spirulina dried by different drying methods drying method temperature c drying time h % phycocyanin recovery wet biomass control 100 cross.
The spirulina platensis cultured with smokestack flue gas from a power plant is valuable. Phycocyanin, which is an important extract of microalgae named spirulina platensis, can inhibit the microsomal lipid peroxidation and hydroxyl. Hippocrates introduction spirulina cyanobacteria has been used as food for centuries by different. Phyocyanin extraction from microalgae spirulina platensis. Over production of phycocyanin pigment in blue green alga. Phycocyanin is a blue colored pigment that belongs to the class of phycobilli proteins found in bluegreen algae. Quantitative analysis of cphycocyanin from spirulina pacifica low temperature method background. Several methods based on chemical, physical, and enzymatic. Research article phycocyanin extraction from spirulina platensis and extract stability under various ph and temperature rachen duangsee 1, natapas phoopat 2 and suwayd ningsanond. Phycocyanin is the responsible component of the blue colour of the bluegreen colour of the spirulina microalgae.
Spirulina protein, benefits, uses, facts, body building. Study of phycocyanin production from spirulina platensis under. In this study a simple protocol was developed for purifying phycocyanin pc from spirulina platensis ccc540 by using ammonium sulphate precipitation, followed by a single step chromatography by using deaecellulose11 and acetate buffer. Phyocyanin extraction from microalgae spirulina platensis assisted by ultrasound irradiation. Bibliography includes bibliographical references and index. Therefore fresh wet biomass was suitable for phycocyanin extraction. Optimization of phycocyanin extraction from spirulina platensis. There are two species, arthrospira platensis and arthrospira maxima. Both native and reduced phycocyanin significantly inhibited peroxyl radicalinduced lipid peroxidation in rat liver microsomes and the inhibition was concentration dependent with an ic50 of 11. Any idea about the highest phycocyanin content in spirulina. Recent developments in production and biotechnological. Growth and immunomodulation properties of this supplement have been widely studied in animals and humans, recently. Phycocyanin is a phytonutrient a protein pigment its natural light is blue and is present in spirulina. Phycocyanin is a pigment protein complex that is heavily sought after for its many health benefits.
It has gained importance in many biotechnological applications in food sciences, therapy, cosmetics and. Phycocyanin in bac enhances the immune system the magic is bac. Autotrophic cultivation of spirulina platensis for co2. Study of phycocyanin production from spirulina platensis. Platensis mainly occurs in asia, kenya and south america, and it is also the most studied species. Nov 03, 2019 the minimum content of phycocyanin in blue green algae as spirulina is more then10% for exemple if you have 100 g of spirulina you can obtein more then 10g of phycocyanine after thawing and. Review article medical application of spirulina platensis derived c phycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3 college of pharmacy, jinan university, guangzhou, china. Phycocyanin extraction in spirulina produced using agricultural. Results were compared to a standard curve of pure cphycocyanin 50. Motile aeromonad septicaemia mas is a major disease in common carp cyprinus carpio caused by aeromonas hydrophila. Growth and immunomodulation properties of this supplement have been.
Phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin. Phycocyanin extraction from cyanobacteria spirulina platensis was optimized using factorial design and response surface techniques. Optimization,purification and characterization of phycocyanin. Spirulina is a simple onecelled microscopic bluegreen algae cyanobacteria. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural institutedeemed university, dindigul, tamilnadu, india corresponding author abstract introduction spirulina is a bluegreen algae owing to the.
Although a number of reports are available for the extraction and purification of phycocyanin from cyanobacterial strains schoenleber et al. The phycocyanin was purified by sephadex g100 and rphplc and protein content was found to be 52. Spirulina contains unusually high amounts of protein, between 55 and 70 percent by dry weight, depending upon the. An important cyanobacteria named spirulina arthrospira plantensis is a micro algae. The experimental data on the study of the antibacterial activity of purified phycocyanin, a proteinbound pigment isolated from bluegreen alga, spirulina platensis nordstedt geitler, oscillatoriaceae are generalized and it was shown that phycocyanin was able to markedly inhibit the growth of drug resistant bacteria escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and. Phycocyanin is one of the major pigment constituents of spirulina, a microalgae used in many countries as a dietary supplement. Cphycocyanin cpc is a natural blue colored protein of spirulina platensis. Antiinflammatory and antihyperalgesic activity of cphycocyanin.
What is phycocianin aka blue spirulina spirulina glossary. Spirulina platensis and its contents have been linked to a nutritional component in both human and animal health and welfare. Herein we report an optimization study of various phycocyanin extraction methods from spirulina platensis cyanobacterium biomass. The basic biochemical composition of spirulina can be summarized as follows. Previous studies have demonstrated the potential health benefits of this natural pigment. An extensive range of pigments including phycobiliproteins are present in algae.
This study aimed to evaluate the performance of phycocyanin immunostimulant extracted from spirulina platensis to control mas disease in common carp. Cyanobacterium strains spirulina platensis cftri is a local isolate, main. The aim of this work was to investigate the production of phycocyanin by spirulina platensis under different spectra of light. Spirulina platensis at high nitrogen level gave highest percentage of total phycocyanin 9. B four photobioreactors 50 l tanks with initial volumes of 20 l of s. Cphycocyanin cpc, a biliprotein found in blue green algae, such as spirulina platensis, is often used as a dietary. Crystal structure of a lightharvesting protein cphycocyanin from spirulina platensis. Rphycocyanin has an absorption maxima at 533 and 544 nm.
It is a complete protein, containing all essential amino acids, though with reduced amounts of methionine, cystine. Among many of spirulina bioactive components, blue protein cphycocyanin and its. Cphycocyanin cpc, a kind of blue protein isolated from spirulina platensis, can ameliorate hyperglycemia, but its effects on gluconeogenesis and glycogenesis are unknown. Cultivation of spirulina platensis is investigated under different conditions. Wound healing effects of cphycocyanin isolated from spirulina platensis. A wide variety of spirulina phycocyanin extract options are available to you, such as part, packaging, and form. Spirulina denaturation and gel formation is therefore a complex phenomenon chronakis, 2001. Improving the biomass productivity and phycocyanin concentration by mixotrophic cultivation of arthrospira platensis master thesis, june 2016. Optimization,purification and characterization of p hycocyanin from spirulina platensis suman saran 1, neha puri 2, nakuleshwar dut jasuja 3, manoj kumar 4 and gaurav sharma 5 1,3,4,5 school of. Growth and phycocyanin formation of spirulina platensis in. Asian journal of food and agroindustry issn 19063040 available online at. Spirulina health benefits theres many health benefits associated with spirulina because of the nutrients found in spirulina such as protein, betacarotene, antioxidants, phycocyanin, chlorophyll, and other vitamins and minerals. Spirulina platensis dried flakes spireat were obtained from algaria slr italy and stored at 4 c in the dark.
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